Summary

This topic demonstrates how to attach a non-peptide structure, e.g. a linker or a lipid, to a peptide sequence.



Step by Step

Drag the mono-substituted terminal atom of a non-peptide structure over the sequence to connect it to. As soon as the mouse enters the sequence rectangle, all available amino acid attachment points are highlighted.


Drop the atom on the desired location. The dropped atom can behave in two ways to form the connection, depending on the selected amino acid attachment center.


  • Attachment center is COOH  →  The dropped atom acts as a nucleophile, performing a formal condensation to form an ester, amide, etc. Example: Bu-SH + HO(O)C-sequence -> BuS(O)C-sequence
  • Attachment center is SH, OH, NHx  →  The dropped atom is replaced by the attachment center atom. Example: Et-Br + HS-Sequence> Et-S-sequence.


A dialog now lets you specify the attachment point on the selected amino acid.



After clicking OK, the connection is created. Please note that an ester was formed in this example, according to the above connection rule.